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Chao, W., Kolski-Andreaco, A. June 2016 - This Month in JoVE: Blueberry Analysis, Impact Testing Football Helmets, Stencil Micropatterning of Stem Cells, and a Test for Soil Plasticity. J. Vis. Exp. (112), e5784, (2016).
In JoVE Chemistry, just in time for blueberry season, we feature a method for predicting the constituents of blueberries while keeping them intact. Bai et al. use near-infrared spectroscopy to obtain spectra of individual blueberries, then use high-performance liquid chromatography (HPLC) to measure their actual contents. They build models that use the near-infrared spectra to predict the levels of sugar, the organic acids that impart tartness, and the anthocyanins that have antioxidant properties. This method aids the selection and distribution of only the most delicious blueberries with guaranteed qualities.
JoVE Engineering includes a test protocol that simulates on-field impacts of football helmets to help reduce the risks of head injuries. Current performance standards for football helmet certification don't require the faceguard to be attached during impact tests; however, attaching a faceguard dramatically changes the helmet's mechanical properties. Therefore, Rush et al. propose a modified test that includes the faceguard. They also test two new helmet impact locations. This method provides more robust helmet safety standards that will drive safer helmet designs.
In JoVE Developmental Biology, human pluripotent stem cells (hPSCs) can differentiate into all three developmental germ layers. However, in vitro differentiation of hPSCs tends to be a disorganized process. This month, Sahni et al. describe stencil micropatterning-a method that can spatially control stem cell microenvironments and organize their differentiation fates. This elegant method provides a valuable experimental model for tissue organization and patterning in embryonic development.
In JoVE Environment, soil plasticity is a concept inspired by the craft of pottery. It refers to the clay-like behavior of certain soils at particular water content levels. The plastic limit of soil is an important parameter in geotechnology, but traditional assessment methods are highly subjective. Thus, Moreno-Maroto and Alonso-Azcárate present an alternative method based on objective measures. This method allows the plastic limit of soil to be obtained with clear and objective criteria.
G. Alston Rush1,2, R. Prabhu1, Gus A. Rush III3, Lakiesha N. Williams1, M. F. Horstemeyer2,4
1Agricultural and Biological Engineering, Mississippi State University, 2Center for Advanced Vehicular Systems, Mississippi State University, 3Rush Sport Medical, 4Mechanical Engineering, Mississippi State University
This article provides a novel technique to assess the performance characteristics of American football helmets by inclusion of faceguards during NOCSAE Standard drop tests. Additionally, two more impact locations are proposed to be added to the NOCSAE certification.
Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates
Geetika Sahni*1, Jun Yuan*1, Yi-Chin Toh1,2
1Department of Biomedical Engineering, National University of Singapore, 2Singapore Institute of Neurotechnology (SINAPSE), National University of Singapore
Human pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns; although this requires the presentation of spatial environmental gradients. We present stencil micropatterning as a simple and robust method to generate biochemical and mechanical gradients for controlling hPSC differentiation patterns.
José Manuel Moreno-Maroto, Jacinto Alonso-Azcárate
Department of Physical Chemistry, Faculty of Environmental Sciences and Biochemistry, University of Castilla-La Mancha
The traditional standardized test for determining the plastic limit in soils is performed by hand, and the result varies depending on the operator. An alternative method based on bending measurements is presented in this study. This allows the plastic limit to be obtained with a clear and objective criterion.
Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements
Wenming Bai1, Norio Yoshimura1, Masao Takayanagi1, Jingai Che2, Naomi Horiuchi2, Isao Ogiwara3
1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 2Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3Institute of Agriculture, Tokyo University of Agriculture and Technology
We present here a protocol to construct and validate models for nondestructive prediction of total sugar, total organic acid, and total anthocyanin content in individual blueberries by near-infrared spectroscopy.
No conflicts of interest declared.