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JoVE Journal Neuroscience
En invasiv metode for aktivering av musen Dentate Gyrus av høyfrekvente stimulering
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Journal (Neuroscience)
Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture
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Journal (Neuroscience)
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En invasiv metode for aktivering av musen Dentate Gyrus av høyfrekvente stimulering

Article DOI: 10.3791/57857-v • 12:26 min • June 2nd, 2018
June 2nd, 2018 •
1, 1,2
1Department of Neurobiology, Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University

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Denne protokollen viser hvordan du setter opp en pålitelig HFS metode i mus. Nerveceller i hele den hippocampus dentate gyrus stimulert elektrisk av HFS direkte og indirekte i vivo. Neuronal aktivitet og molekylære signalene er undersøkt av c-fos og Notch1 immunofluorescent flekker, henholdsvis; neurogenesis er kvantifisert ved bromodeoxyuridine merking analysen.

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Nevrovitenskap problemet 136 høyfrekvens stimulering dentate gyrus neuronal aktivitet neurogenesis BrdU merking
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