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Journal / Biochemistry / Fluorescens Live-celle billeddannelse af den…
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Biochemistry

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JoVE Journal Biochemistry
Fluorescens Live-celle billeddannelse af den komplette Vegetative cellecyklus Slow-voksende sociale bakterien Myxococcus xanthus
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Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
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Fluorescens Live-celle billeddannelse af den komplette Vegetative cellecyklus Slow-voksende sociale bakterien Myxococcus xanthus

Article DOI: 10.3791/57860-v • 11:45 min • June 20th, 2018
June 20th, 2018 •
1, 1
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

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Bakterieceller er rumligt yderst organiseret. For at følge denne organisation over tid i langsomt voksende Myxococcus xanthus celler, blev en set-up for fluorescens live-celle imaging med høj spatiotemporelle opløsning over flere generationer udviklet. Brug denne metode, kunne spatiotemporelle dynamics af vigtige proteiner til kromosom segregation og celledeling bestemmes.

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Biokemi sag 136 Myxococcus xanthus celledeling celle cyklus time-lapse mikroskopi FtsZ kromosom segregation spatio-temporale organisation
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