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Journal / Biochemistry / 校正と単一のヌクレオチドの拡張子と MALDI-TOF 質量分析を用いた DNA…
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Biochemistry

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JoVE Journal Biochemistry
校正と単一のヌクレオチドの拡張子と MALDI-TOF 質量分析を用いた DNA 修復アッセイ
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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Journal (Biochemistry)
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校正と単一のヌクレオチドの拡張子と MALDI-TOF 質量分析を用いた DNA 修復アッセイ

Article DOI: 10.3791/57862-v • 11:08 min • June 19th, 2018
June 19th, 2018 •
*1,2, *3, 1, 1, 1, 2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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非標識、ラジオ-同位体 DNA ポリメラーゼの校正と DNA 修復アッセイ法は高分解能 MALDI-TOF 質量分析法および単一のヌクレオチドの拡張戦略を使用して開発されました。アッセイは、非常に特定、簡単、迅速かつ簡単に校正を実行し、修復するパッチ 9-ヌクレオチドより短いを証明しました。

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生化学、問題 136 の DNA ポリメラーゼ校正、DNA 修復、DNA 複製エラー、DNA 不一致 dideoxyribonucleotide 三リン酸一塩拡張子 MALDI-TOF 質量分析法
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