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Journal / Biochemistry / Schnelle Isolierung von Mitoribosome von HEK…
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Biochemistry

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JoVE Journal Biochemistry
Schnelle Isolierung von Mitoribosome von HEK Zellen
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Schnelle Isolierung von Mitoribosome von HEK Zellen

Article DOI: 10.3791/57877-v • 09:33 min • October 4th, 2018
October 4th, 2018 •
1, 1, 1, 1
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

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Mitochondrien haben Ribosomen spezialisiert, die von bakteriellen und zytoplasmatischen Gegenstücke abwich. Hier zeigen wir, wie Mitoribosomes ihre native Fach in HEK Zellen entnommen werden können. Die Methode besteht darin, Isolierung von Mitochondrien aus Aussetzung Zellen und konsequente Reinigung der Mitoribosomes.

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Biochemie Ausgabe 140 Mitochondrien Übersetzung Ribosom Cryo-EM Proteinsynthese Biochemie
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