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Developmental Biology
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JoVE Journal Developmental Biology
En kvantitativ måling af reaktive ilt arter og gulne-associerede sekretoriske fænotype i normale humane fibroblaster under onkogen-induceret gulne
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En kvantitativ måling af reaktive ilt arter og gulne-associerede sekretoriske fænotype i normale humane fibroblaster under onkogen-induceret gulne

Article DOI: 10.3791/57890-v • 14:00 min • August 12th, 2018
August 12th, 2018 •
*1,2, *1,2, 1,2
1Department of Biochemistry, College of Medicine, Dong-A University, 2Peripheral Neuropathy Research Center, College of Medicine, Dong-A University
* These authors contributed equally

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Intracellulære ROS har vist sig at spille en vigtig rolle i induktion af cellulære ældning. Her, beskriver vi en følsom analyse for kvantificering ROS niveauer under cellulære ældning. Vi leverer også protokoller for at vurdere den gulne-associerede sekretoriske fænotype, som efter sigende bidrager til forskellige aldersrelaterede dysfunktioner.

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Udviklingsmæssige biologi spørgsmålet 138 cellulære gulne H-Ras ROS SASP IL-6 IL-8 aging
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