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Journal / Biochemistry / Kontrast-samsvarende vaskemiddel i liten vinkel…
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Biochemistry

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JoVE Journal Biochemistry
Kontrast-samsvarende vaskemiddel i liten vinkel Neutron spredning eksperimenter for membran Protein strukturell analyse og Ab Initio modellering
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Kontrast-samsvarende vaskemiddel i liten vinkel Neutron spredning eksperimenter for membran Protein strukturell analyse og Ab Initio modellering

Article DOI: 10.3791/57901-v • 10:27 min • October 21st, 2018
October 21st, 2018 •
1, 2, 1, 1, 2, 1
1Neutron Scattering Division, Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry, Georgia Institute of Technology

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Denne protokollen demonstrerer hvordan å få en lav oppløsning ab initio modell og strukturelle detaljer i et vaskemiddel-solubilized membran protein i løsning med liten vinkel neutron spredning med kontrast-matching av vaskemiddel.

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Biokjemi problemet 140 liten vinkel spredning neutron spredning biofysikk membran proteiner kontrast variasjon surfactant micelle intramembrane aspartyl protease presenilin signalet peptid peptidase
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