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Journal / Biochemistry / Analysere dynamisk Protein komplekser sammen på…
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Biochemistry

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JoVE Journal Biochemistry
Analysere dynamisk Protein komplekser sammen på og løslatt fra Biolayer Interferometry Biosensor massespektrometri og elektronmikroskop
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Journal (Biochemistry)
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An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
Journal (Biochemistry)
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Analysere dynamisk Protein komplekser sammen på og løslatt fra Biolayer Interferometry Biosensor massespektrometri og elektronmikroskop

Article DOI: 10.3791/57902-v • 09:30 min • August 6th, 2018
August 6th, 2018 •
1, 1, 2, 1, 1, 1
1Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, 2Department of Chemistry, Massachusetts Institute of Technology

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Her presenterer vi en protokoll for å overvåke montering og demontering av miltbrann giften bruker biolayer interferometry (BLI). Etter montering/demontering på biosensor overflaten, store protein komplekser er løslatt fra overflaten for visualisering og identifikasjon av komponenter i komplekser med elektronmikroskop og massespektrometri, henholdsvis.

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Biokjemi problemet 138 Biolayer interferometry elektronmikroskop massespektrometri anthrax gift protein komplekser biosensor
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