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Produktion und Visualisierung von bakteriellen Spheroplasts und Protoplasten antimikrobielle Peptide Lokalisierung zu charakterisieren
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Produktion und Visualisierung von bakteriellen Spheroplasts und Protoplasten antimikrobielle Peptide Lokalisierung zu charakterisieren

Article DOI: 10.3791/57904-v • 10:13 min • August 11th, 2018
August 11th, 2018 •
1, 1,2, 2, 1,2, 1,3
1Biochemistry Program, Wellesley College, 2Department of Chemistry, Wellesley College, 3Department of Biological Sciences, Wellesley College

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Hier präsentieren wir ein Protokoll, um Gramnegative Escherichia coli (E. Coli) produzieren Spheroplasts und gram-positiven Bacillus Megatherium (B. Megatherium) Protoplasten deutlich sichtbar zu machen und schnell charakterisieren Peptid-Bakterien Interaktionen. Dies bietet eine systematische Methode zur Membran Lokalisierung und translocating Peptide zu definieren.

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Biologie Ausgabe 138 antimikrobielle Peptid Protoplasten Spheroplast Mikroskopie konfokale Translokation Permeabilisierung
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