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Produktion og visualisering af bakterielle Spheroplasts og Protoplasts til at karakterisere antimikrobielle peptid lokalisering
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Produktion og visualisering af bakterielle Spheroplasts og Protoplasts til at karakterisere antimikrobielle peptid lokalisering

Article DOI: 10.3791/57904-v • 10:13 min • August 11th, 2018
August 11th, 2018 •
1, 1,2, 2, 1,2, 1,3
1Biochemistry Program, Wellesley College, 2Department of Chemistry, Wellesley College, 3Department of Biological Sciences, Wellesley College

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Her præsenterer vi en protokol for at producere gram-negative Escherichia coli (E. coli) spheroplasts og gram-positive Bacillus megaterium (B. megaterium) protoplasts at visualisere, klart og hurtigt karakterisere peptid-bakterier interaktioner. Dette giver en systematisk metode til at definere membran lokalisering og translocating peptider.

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Biologi spørgsmålet 138 antimikrobielle peptid protoplast spheroplast mikroskopi Konfokal omplantning permeabilization
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