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Oppdage Protein Subcellular lokalisering av Green fluorescens Protein merking og 4', 6-Diamidino-2-phenylindole farging i Caenorhabditis elegans
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Oppdage Protein Subcellular lokalisering av Green fluorescens Protein merking og 4', 6-Diamidino-2-phenylindole farging i Caenorhabditis elegans

Article DOI: 10.3791/57914-v • 09:36 min • July 30th, 2018
July 30th, 2018 •
1, 1, 1
1Department of Science, Borough of Manhattan Community College/CUNY

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Denne protokollen demonstrerer hvordan å spore et protein kjernefysiske translokasjon under varmestress med en grønn fluorescens protein (GFP) fusion protein som en markør og 4', 6-diamidino-2-phenylindole (DAPI) flekker. DAPI flekker protokollen er rask og beholder GFP og protein subcellular lokalisering signaler.

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Biologi problemet 137 varmestress subcellular lokalisering endre kjernefysiske lokalisering grønne fluorescens protein DAPI flekker etanol fiksering aceton fiksering
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