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Journal / Developmental Biology / Kemiske Reversion af konventionelle humane…
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JoVE Journal Developmental Biology
Kemiske Reversion af konventionelle humane pluripotente stamceller til en naiv-lignende tilstand med forbedret Multilineage differentiering potens
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Kemiske Reversion af konventionelle humane pluripotente stamceller til en naiv-lignende tilstand med forbedret Multilineage differentiering potens

Article DOI: 10.3791/57921-v • 09:07 min • June 10th, 2018
June 10th, 2018 •
*1, *1, 1, 1
1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering, Johns Hopkins School of Medicine
* These authors contributed equally

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Vi præsenterer en protokol for effektiv, bulk og hurtige kemiske reversion af konventionelle afstamning-pulverlak humane pluripotente stamceller (hPSC) ind i en epigenomically-stabil naive præimplantations epiblast-lignende pluripotente tilstand. Denne metode resulterer i nedsat afstamning-pulverlak genekspression og markant forbedring i styret multilineage differentiering på tværs af et bredt repertoire af konventionelle hPSC linjer.

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Udviklingsmæssige biologi sag 136 naive pluripotency humane pluripotente stamceller differentiering tankyrase hæmning lille molekyle epiblast
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