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Journal / Bioengineering / Um Orbital agitando a cultura de células de…
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Bioengineering

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JoVE Journal Bioengineering
Um Orbital agitando a cultura de células de mamíferos em vasos em forma do produzir agregados uniformes
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Um Orbital agitando a cultura de células de mamíferos em vasos em forma do produzir agregados uniformes

Article DOI: 10.3791/57922-v • 05:40 min • January 7th, 2019
January 7th, 2019 •
1,2, 3, 3, 1,4
1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo

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Aqui nós apresentamos um protocolo para a utilização dos recipientes em forma de O, especializados para culturas de suspensão de agregados celulares, com agitação orbital. As células HEK293 crescidas neste saco mais formam agregados homogêneos do que aquelas cultivadas em vasos de cultura convencional.

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Bioengenharia edição 143 cultura de suspensão orbital tremendo produção em massa pilha mamífera saco de cultura agregado
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