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Journal / Immunology and Infection / Caracterização de subconjuntos de monócitos…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Caracterização de subconjuntos de monócitos humanos por sangue total Flow Cytometry Analysis
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Caracterização de subconjuntos de monócitos humanos por sangue total Flow Cytometry Analysis

Article DOI: 10.3791/57941-v • 09:12 min • October 17th, 2018
October 17th, 2018 •
1,2, 1,2, 3, 4, *1,2, *1,2
1Department of Surgery, Vascular Biology Research Centre, Westmead Hospital, 2Westmead Clinical School, Department of Surgery, The University of Sydney, 3Westmead Research Hub, Westmead Institute for Medical Research, 4Institute for Clinical Pathology and Medical Research, Westmead Hospital
* These authors contributed equally

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Aqui nós apresentamos um protocolo para caracterizar subconjuntos de monócitos por citometria de fluxo de sangue total. Isso inclui como portão os subconjuntos e avaliar sua expressão de marcadores de superfície de estrutura de tópicos e dando um exemplo da avaliação da expressão de marcadores M2 (anti-inflamatório) e M1 (inflamatória).

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Imunologia Imunologia e infecção questão 140 monócitos citometria de fluxo inflamação retenção de monócitos expressão do marcador de monócitos macrófagos aterosclerose
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