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Digital PCR for kvantifisering sirkulerer MicroRNAs i akutt hjerteinfarkt og hjerte-og karsykdommer
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Digital PCR for kvantifisering sirkulerer MicroRNAs i akutt hjerteinfarkt og hjerte-og karsykdommer

Article DOI: 10.3791/57950-v • 04:41 min • July 3rd, 2018
July 3rd, 2018 •
1, 1,2, 3, 1, 1, 1, 1, 1,4, 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

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Sirkulerende microRNAs har vist lovende som biomarkers for kardiovaskulære sykdommer og akutt myokardisk infarkt. I denne studien beskriver vi en protokoll for miRNA utvinning, omvendt transkripsjon og digital PCR for den absolutte kvantifiseringen av miRNAs i serum av pasienter med hjerte-og karsykdommer.

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Medisin problemet 137 hjerteinfarkt hjerte-og karsykdommer digital PCR microRNAs koronarsykdom serum biomarkers
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