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PCR digitale per quantificare circolanti microRNA nell'infarto miocardico acuto e la malattia cardiovascolare
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PCR digitale per quantificare circolanti microRNA nell'infarto miocardico acuto e la malattia cardiovascolare

Article DOI: 10.3791/57950-v • 04:41 min • July 3rd, 2018
July 3rd, 2018 •
1, 1,2, 3, 1, 1, 1, 1, 1,4, 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

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MicroRNA circolanti hanno indicato la promessa come biomarcatori per malattie cardiovascolari e infarti miocardici acuti. In questo studio, descriviamo un protocollo per l'estrazione di miRNA, trascrizione d'inversione e digitali PCR per la quantificazione assoluta dei miRNA nel siero dei pazienti con la malattia cardiovascolare.

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Medicina problema 137 infarto del miocardio malattia cardiovascolare PCR digitale microRNA malattia dell'arteria coronaria biomarcatori del siero
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