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PCR digital para quantificar circulando MicroRNAs no infarto agudo do miocárdio e doença Cardiovascular
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PCR digital para quantificar circulando MicroRNAs no infarto agudo do miocárdio e doença Cardiovascular

Article DOI: 10.3791/57950-v • 04:41 min • July 3rd, 2018
July 3rd, 2018 •
1, 1,2, 3, 1, 1, 1, 1, 1,4, 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

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MicroRNAs circulantes têm mostrado promessa como biomarcadores para doenças cardiovasculares e aguda do miocárdio. Neste estudo, descrevemos um protocolo para extração de miRNA, a transcrição reversa e PCR digital para a quantificação absoluta dos miRNAs no soro de pacientes com doença cardiovascular.

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Medicina edição 137 infarto do miocárdio doença cardiovascular PCR digital microRNAs doença arterial coronariana biomarcadores de soro
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