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Laktat Dehydrogenase lagring analysen-En enkel og pålitelig metode å avgjøre Bulk Autophagic lagring aktivitet i pattedyrceller
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Laktat Dehydrogenase lagring analysen-En enkel og pålitelig metode å avgjøre Bulk Autophagic lagring aktivitet i pattedyrceller

Article DOI: 10.3791/57971-v • 09:34 min • July 27th, 2018
July 27th, 2018 •
*1, *1, 1
1The Autophagy Team, Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo
* These authors contributed equally

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Her er en enkel og godt validert protokoll for å måle bulk autophagic lagring aktivitet i pattedyrceller beskrevet. Metoden er basert på kvantifisere andelen av laktat dehydrogenase (LDH) i sedimentable celle fraksjoner sammenlignet med totale mobilnettet LDH nivåer.

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Biologi problemet 137 LDH laktat dehydrogenase lagring autophagy autophagic Last phagophore autophagosome Bafilomycin A1 LC3
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