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JoVE Journal Developmental Biology
Análises de célula única Transcriptomic de células endócrinas no pâncreas de rato
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Análises de célula única Transcriptomic de células endócrinas no pâncreas de rato

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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Nós descrevemos um método para o isolamento de células endócrinas de pancreases embrionárias, Neonatais e pós-natal, seguidos por sequenciação do ARN de célula única. Esse método permite que as análises do desenvolvimento de linhagem endócrina do pâncreas, heterogeneidade e transcriptomic dinâmica de células.

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Biologia do desenvolvimento edição 139 pâncreas endócrino cells células β célula única-a sequenciação do ARN expressão gênica heterogeneidade celular
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