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JoVE Journal Developmental Biology
Single-cell Transcriptomic analyser av mus bukspottkörtelns endokrina celler
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Single-cell Transcriptomic analyser av mus bukspottkörtelns endokrina celler

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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Vi beskriver en metod för isolering av endokrina celler från embryon, neonatal och postnatal bukspottkörtlar följt av encelliga RNA-sekvensering. Denna metod tillåter analyser av pankreas endokrina härstamning utveckling, cell heterogenitet och transcriptomic dynamik.

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Utvecklingsbiologi fråga 139 pankreas endokrina celler β-cellen Single-cell RNA-sekvensering genuttryck cellulära heterogenitet
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