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Journal / Genetics / 用于下一代测序的近全长 HIV-1 Proviruses…
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Genetics

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JoVE Journal Genetics
用于下一代测序的近全长 HIV-1 Proviruses 的扩增
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用于下一代测序的近全长 HIV-1 Proviruses 的扩增

Article DOI: 10.3791/58016-v • 10:18 min • October 16th, 2018
October 16th, 2018 •
1, 1, 1, 1
1Centre for Virus Research, The Westmead Institute for Medical Research, University of Sydney

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全长单个前病毒测序 (翻转) 为单、近全长 (完好和有缺陷) HIV-1 proviruses 的扩增和测序提供高效和高通量的方法, 并可确定其电位复制能力。翻转克服了先前用于序列化潜伏 HIV-1 油藏的检测的局限性。

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遗传学 问题 140 下一代测序 HIV-1 hiv proviruses 单前病毒测序 hiv DNA 潜伏期 复制能力 抗逆转录病毒疗法 hiv 病毒库 综合 hiv
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