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Versterking van in de buurt van Full-length HIV-1 Proviruses voor Next-Generation Sequencing
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Versterking van in de buurt van Full-length HIV-1 Proviruses voor Next-Generation Sequencing

Article DOI: 10.3791/58016-v • 10:18 min • October 16th, 2018
October 16th, 2018 •
1, 1, 1, 1
1Centre for Virus Research, The Westmead Institute for Medical Research, University of Sydney

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Full-length individuele proviraal sequencing (FLIPS) een efficiënte en hoog-productie methode voorziet in de versterking en sequentiebepaling van één, in de buurt van full-length (intact en defecte) HIV-1 proviruses en zorgt voor de bepaling van hun potentieel replicatie-competentie. FLIPS overwint beperkingen van eerdere tests ontworpen om de volgorde van het latente reservoir van HIV-1.

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Genetica kwestie 140 Next-generation sequencing HIV-1 HIV sequencing proviruses één-proviraal HIV-DNA replicatie-bekwaamheid antiretrovirale therapie latency HIV reservoir geïntegreerde HIV
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