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Journal / Genetics / Forstærkning af nær fuld længde HIV-1 provirus…
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Genetics

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Forstærkning af nær fuld længde HIV-1 provirus for næste Generation Sequencing

Article DOI: 10.3791/58016-v • 10:18 min • October 16th, 2018
October 16th, 2018 •
1, 1, 1, 1
1Centre for Virus Research, The Westmead Institute for Medical Research, University of Sydney

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Fuld længde individuelle proviral sekventering (FLIPS) giver en effektiv og høj overførselshastighed metode til forstærkning og sekventering af enkelt, nær fuld længde (intakt og defekte) HIV-1 provirus og giver mulighed for bestemmelse af deres potentiale replikering-kompetence. FLIPS overvinder begrænsningerne af tidligere assays designet til sekvens latent HIV-1 reservoiret.

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Genetik spørgsmål 140 næste generation sequencing HIV-1 HIV sekventering provirus single-proviral HIV DNA ventetid replikation-kompetence antiretroviral behandling HIV reservoir integreret HIV
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