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Journal / Biology / 研究核苷酸合并: 酵母基因组 Ribonucleotides…
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研究核苷酸合并
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研究核苷酸合并: 酵母基因组 Ribonucleotides 的链特异检测及核苷酸诱变的检测

Article DOI: 10.3791/58020-v • 09:04 min • July 26th, 2018
July 26th, 2018 •
*1, *1, 1
1Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, DHHS
* These authors contributed equally

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Ribonucleotides 是在核 DNA 复制过程中纳入基因组的最丰富的非规范核苷酸之一。如果没有正确去除, ribonucleotides 会导致 DNA 损伤和突变。在这里, 我们提出了两个实验方法, 用于评估核苷酸的丰度纳入 DNA 及其诱变效应。

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生物 问题 137 核苷酸并网 DNA 复制 RNase H2 碱性凝胶电泳 波动测试 诱变
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