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Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
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Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Article DOI: 10.3791/58041-v • 08:44 min • August 3rd, 2018
August 3rd, 2018 •
1, 1, 1, 1,2
1Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University

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We have developed a simple and adaptable workflow to extract quantitative data from fluorescence-imaging-based cell biological studies of protein aggregation and autophagic flux in the central nervous system of Drosophila models of neurodegeneration.

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Quantitative Cell Biology Neurodegeneration Drosophila Fluorescently Tagged Proteins ImageJ Unbiased Analysis Adaptable Image Analysis Workflow Selection Bias Sampling Power Reproducibility Proteinaceous Puncta Membrane-bound Compartments Mechanistic Understanding Lamina Dissection Forceps Retina Removal Fixative PBS Plus Triton X-100 Primary Antibody
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