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JoVE Journal Neuroscience
Biologia cellulare quantitativa della neurodegenerazione in Drosophila attraverso analisi imparziale delle proteine fluorescente contrassegnate usando ImageJ
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Biologia cellulare quantitativa della neurodegenerazione in Drosophila attraverso analisi imparziale delle proteine fluorescente contrassegnate usando ImageJ

Article DOI: 10.3791/58041-v • 08:44 min • August 3rd, 2018
August 3rd, 2018 •
1, 1, 1, 1,2
1Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, 2School of Pharmacy, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Yantai University

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Abbiamo sviluppato un flusso di lavoro semplice e adattabile per estrarre dati quantitativi da studi biologici basati su formazione immagine di fluorescenza delle cellule di aggregazione proteica e flusso autofagico nel sistema nervoso centrale di Drosophila modelli di neurodegenerazione.

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L'autofagia aggregata neuroscienze problema 138 ImageJ segmentazione raziometrico neurodegenerazione Drosophila il cervello
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