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Optisk analysen for Synaptic Vesicle resirkulering i kulturperler Neurons Overexpressing Presynaptic proteiner
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Optisk analysen for Synaptic Vesicle resirkulering i kulturperler Neurons Overexpressing Presynaptic proteiner

Article DOI: 10.3791/58043-v • 09:33 min • June 26th, 2018
June 26th, 2018 •
1, 1, 1, 1
1Institute for Anatomy and Embryology, University Medical Centre Göttingen

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Vi beskriver en optisk analysen for synaptic vesicle (SV) resirkulering i kulturperler neurons. Kombinere denne protokollen med dobbel transfection å uttrykke en presynaptic markør og protein rundt tillater oss å finne presynaptic steder, deres synaptic vesicle resirkulering kapasitet, og bestemme rollen protein av interesse.

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Nevrovitenskap problemet 136 Synaptic blemmer resirkulering synapser neurons Synaptotagmin-1 hippocampus rotte cellekultur
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