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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikation og isolering af Oligopotent og afstamning-begået myeloide ophav fra mus knoglemarv
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Identifikation og isolering af Oligopotent og afstamning-begået myeloide ophav fra mus knoglemarv

Article DOI: 10.3791/58061-v • 07:21 min • July 29th, 2018
July 29th, 2018 •
1,2, 1,2
1Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 2Research Division of Immunology, Cedars-Sinai Medical Center

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Vi demonstrere, hvordan til at identificere og isolere 6 delmængder af myeloide stamceller fra murine knoglemarven ved hjælp af en kombination af magnetiske og fluorescens sortering (MACS og FACS). Denne protokol kan bruges til i vitro kultur assays (methylcellulose eller flydende kulturer), i vivo adoptiv overførsel eksperimenter og RNA/protein analyser.

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Immunologi og infektion sag 137 Myeloid progenitorceller fælles myeloide stamceller (CMP) granulocyt-monocyt stamfader (GMP) monocyt-DC stamfader (MDP) granulocyt stamfader (GP) monocyt stamfader (MP eller cMoP) knoglemarv magnetisk aktiveret celle sortering (MLA) fluorescens-aktiveret celle sortering (FACS)
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