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JoVE Journal
Immunology and Infection

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En høj overførselshastighed Cre-Lox aktiveret Viral membran Fusion analyse for at identificere hæmmere af HIV-1 Viral membran Fusion
 
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En høj overførselshastighed Cre-Lox aktiveret Viral membran Fusion analyse for at identificere hæmmere af HIV-1 Viral membran Fusion

Article DOI: 10.3791/58074-v 07:22 min August 14th, 2018
August 14th, 2018

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Summary

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Vi beskriver en celle-baseret analyse for at rapportere om HIV-1 fusion via udtryk for grøn fluorescerende proteiner kan påvises ved flow flowcytometri eller Fluorescens mikroskopi. Det kan bruges til at teste hæmmere af viral entry (specielt på trinnet fusion) i celle-fri og celle til celle infektion systemer.

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Immunologi og infektion spørgsmålet 138 HIV-1 indgang fusion screening Cre recombinase celle til celle transmission purinergic receptorer
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