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Journal / Immunology and Infection / 体外蛍光顕微鏡による犬の好中球細胞外トラップ形成:…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
体外蛍光顕微鏡による犬の好中球細胞外トラップ形成
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
Journal (Immunology and Infection)
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
Journal (Immunology and Infection)
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
 
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体外蛍光顕微鏡による犬の好中球細胞外トラップ形成: ダイナミックな定量的分析

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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全血からの犬好中球を分離し、蛍光顕微鏡を用いたライブ好中球におけるネットの形成を視覚化する方法について述べる。ネット形成とシトルリン化ヒストン H3 を定量化するプロトコルについて述べる、蛍光顕微鏡を用いた (citH3) 式。

Tags

免疫学、感染症、問題 138、蛍光顕微鏡、敗血症、顆粒球の分離、ヒストン、シトルリン、ペプチジルアルギニンデイミナーゼ アルギニンデイミナーゼ
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