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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Formación de la trampa extracelular neutrófilo canino
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In Vitro Formación de la trampa extracelular neutrófilo canino: Análisis dinámico y cuantitativo por microscopía de fluorescencia

Article DOI: 10.3791/58083-v • 09:45 min • August 24th, 2018
August 24th, 2018 •
1, 2
1Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 2Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis

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Se describen métodos para aislar caninas neutrófilos de la sangre entera y visualizar la formación neta en vivo neutrófilos mediante microscopía de fluorescencia. También se describe son protocolos para cuantificar la formación neta y citrulinado histona H3 (citH3) expresión usando microscopia de la inmunofluorescencia.

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Inmunología e infección número 138 microscopia de la inmunofluorescencia sepsis aislamiento de colonias de granulocitos histona citrullination peptidylarginine deiminase
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