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Journal / Immunology and Infection / In Vitro Diferenciación del Factor del…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Diferenciación del Factor del Granulocyte-macrófago-estimulante de colonias (GM-CSF) de ratón-producción de linfocitos de T (THGM)
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In Vitro Diferenciación del Factor del Granulocyte-macrófago-estimulante de colonias (GM-CSF) de ratón-producción de linfocitos de T (THGM)

Article DOI: 10.3791/58087-v • 10:28 min • September 10th, 2018
September 10th, 2018 •
1,2,3, 3, 1,2
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Immunology Programme, Life Sciences Institute, National University of Singapore, 3Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

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Aquí, presentamos un protocolo para distinguir murino granulocyte-macrophage-colony-stimulating-factor-producing T (THGM) linfocitos de células T CD4 + de ingenuo, incluyendo el aislamiento de las células CD4 + T de ingenuo, diferenciación de THGM, y Análisis de las células THGM diferenciadas. Este método puede ser aplicado a los estudios de la regulación y función de las células THGM.

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Inmunología e infección número 139 GM deHT célula T helper celular diferenciación GM-CSF ingenuo T neuroinflamación citoquinas inflamatorias
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