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JoVE Journal Genetics
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Article DOI: 10.3791/58089-v • 07:56 min • November 16th, 2018
November 16th, 2018 •
*1,2, *3, 3, 3, 1,2, 3
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty at Rostock University, 3Department of Operative Dentistry and Periodontology, Rostock University Medical Center
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes the transient genetic engineering of dental stem cells extracted from the human dental follicle. The applied non-viral modification strategy may become a basis for the improvement of therapeutic stem cell products.

Tags

Isolation Characterization MicroRNA-based Genetic Modification Human Dental Follicle Stem Cells Regenerative Medicine Therapeutic Properties MicroRNA Introduction Stable Genome Integration Enzymatic Digestion Collagenase Type I Dispase II Basal Medium Antibiotic Agent Sterile Petri Dish PBSPSG Solution Extracted Tooth Follicle Sterile Scalpel Minced Tissue Conical Centrifuge Tube
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