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Medição de Mitophagy sensível pelo repórter fluorescentes Flow Cytometry usando o pH-dependente mt-Keima

Article DOI: 10.3791/58099-v • 09:14 min • August 12th, 2018
August 12th, 2018 •
*1,2, *1,2, 3, 1,2
1Peripheral Neuropathy Research Center, College of Medicine, Dong-A University, 2Department of Biochemistry, College of Medicine, Dong-A University, 3Aging Institute, Department of Medicine, University of Pittsburgh School of Medicine
* These authors contributed equally

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Mitophagy, a degradação seletiva da mitocôndria, tem sido implicada na homeostase mitocondrial e é desregulamentado em várias doenças humanas. No entanto, convenientes métodos experimentais para medir a atividade de mitophagy são muito limitados. Aqui, nós fornecemos um ensaio sensível para medir a atividade de mitophagy usando citometria de fluxo.

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Biologia edição 138 Mitophagy mitocôndrias mt-Keima citometria de fluxo proteína fluorescente sonda de pH-dependente
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