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JoVE Journal Biochemistry
Funksjonell karakteristikk av Carboxylesterases i insektmiddel resistente hus fluer, Musca Domestica
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Funksjonell karakteristikk av Carboxylesterases i insektmiddel resistente hus fluer, Musca Domestica

Article DOI: 10.3791/58106-v • 08:17 min • August 23rd, 2018
August 23rd, 2018 •
1, 1
1Department of Entomology and Plant Pathology, Auburn University

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Her presenterer vi en protokoll for å produsere huset fly carboxylesterase proteiner i vitro med en baculovirus mediert insekt celle uttrykk og senere funksjonelt karakterisere deres roller i metabolizing permethrin, dermed overdragelse pyrethroid motstand ved å gjennomføre cellebasert MTT analysen og i vitro metabolske studier.

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Biokjemi problemet 138 insektmiddel motstand carboxylesterases baculovirus-mediert insekt celle uttrykk systemet cytotoksisitet analysen i vitro metabolisme analysen
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