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JoVE Journal Biochemistry
Funktionell karakterisering av Carboxylesterases i insektsmedel resistenta hus flugor, Musca Domestica
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Journal (Biochemistry)
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Journal (Biochemistry)
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
 
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Funktionell karakterisering av Carboxylesterases i insektsmedel resistenta hus flugor, Musca Domestica

Article DOI: 10.3791/58106-v • 08:17 min • August 23rd, 2018
August 23rd, 2018 •
1, 1
1Department of Entomology and Plant Pathology, Auburn University

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Här presenterar vi ett protokoll för att producera house fly karboxylesteras proteiner i vitro med en baculoviridae medierad insekt cell uttryck systemet och senare funktionellt präglar deras roller i Fettnedbrytande permetrin, därmed ger pyretroidbaserade motstånd av cellbaserade MTT assay och in vitro- metaboliska studier.

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Biokemi fråga 138 insektsmedel motstånd carboxylesterases baculoviridae-medierad insekt cell uttryck systemet cytotoxicitet analys in vitro ämnesomsättning analys
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