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JoVE Journal
Biochemistry

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基于板块的秀丽线虫规模化栽培
 
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基于板块的秀丽线虫规模化栽培: 糖尿病代谢变化研究的样品制备

Article DOI: 10.3791/58117-v 05:08 min August 24th, 2018
August 24th, 2018

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Summary

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本协议描述了一种在固体培养基上大规模培养秀丽线虫的方法。作为液体培养的替代品, 该协议允许在板基培养条件下获得不同尺度的参数。这增加了结果的可比性通过省略液体和固体媒介文化之间的形态学和新陈代谢的区别。

Tags

生物化学 问题 138 C. 线虫 大型养殖 反应性代谢产物 晚期糖基化终产物 活性氧 蛋白质提取物 全身裂解 板培养 洗涤规程 质谱 糖尿病并发症
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