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Journal / Genetics / Proteina endogena Tagging in umani hanno indotto…
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Genetics
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JoVE Journal Genetics
Proteina endogena Tagging in umani hanno indotto le cellule staminali pluripotenti utilizzando CRISPR/Cas9
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Proteina endogena Tagging in umani hanno indotto le cellule staminali pluripotenti utilizzando CRISPR/Cas9

Article DOI: 10.3791/58130-v • 14:49 min • August 25th, 2018
August 25th, 2018 •
1, 1, 1, 1, 1, 1
1Allen Institute for Cell Science

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Descritto qui è un protocollo per l'etichettatura in modo endogeno espresse proteine con tag fluorescente in cellule staminali pluripotenti indotte umane utilizzando CRISPR/Cas9. Celle putativamente modificate sono arricchite da ordinamento di attivate la fluorescenza delle cellule e linee clonali della cellula vengono generate.

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Genetica cellule staminali problema 138 hiPSC biologia molecolare CRISPR Cas9 ingegneria del genoma Gene Knock-in Gene Tagging proteina fluorescente
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