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JoVE Journal Immunology and Infection
Valutazione dell'interfaccia sinaptica primaria umana delle cellule di T dal sangue periferico e tessuto linfoide
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Valutazione dell'interfaccia sinaptica primaria umana delle cellule di T dal sangue periferico e tessuto linfoide

Article DOI: 10.3791/58143-v • 06:27 min • July 30th, 2018
July 30th, 2018 •
1, 1, 2,3, 2, 4
1Department of Microbiology and Immunology, Thomas Jefferson University, 2Department of Microbiology and Institute for Immunology, Perelman School of Medicine, University of Pennsylvania, 3Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital Huddinge, 4Departments of Microbiology and Immunology and Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University

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Il protocollo descrive una tecnica per studiare la capacità delle cellule T umane policlonali primarie per formare sinaptiche interfacce utilizzando lipidici planare. Usiamo questa tecnica per dimostrare la capacità di formazione di sinapsi differenziale delle cellule T umane primarie derivata da linfonodi e nel sangue periferico.

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Immunologia e infezione problema 137 cellule T umane primarie sangue periferico i linfonodi planare lipidici struttura interfaccia sinaptica
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