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Journal / Medicine / En effektiv Sieving metode til at isolere intakt…
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JoVE Journal Medicine
En effektiv Sieving metode til at isolere intakt Glomeruli fra voksen rotte nyre
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En effektiv Sieving metode til at isolere intakt Glomeruli fra voksen rotte nyre

Article DOI: 10.3791/58162-v • 10:14 min • November 1st, 2018
November 1st, 2018 •
*1, *1, 2, 1
1Division of Renal-Electrolyte, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh
* These authors contributed equally

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Hovedfokus i denne protokol er effektivt isolere levedygtige primære glomeruli kulturer med minimal forurenende stoffer til brug i en lang række downstream applikationer. Isolerede glomeruli bevarer strukturelle relationer mellem komponent celletyper og kan være kulturperler ex vivo for kort tid.

Tags

Medicin sag 141 nyre foden proces Effacement glomerulær skade proteinuri glomerulær permeabilitet Protamine sulfat Renal patologi glomerulær sygdom
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