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Journal / Medicine / Effektiv Sieving metode å isolere intakt…
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Effektiv Sieving metode å isolere intakt Glomeruli fra voksen rotte nyre
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Effektiv Sieving metode å isolere intakt Glomeruli fra voksen rotte nyre

Article DOI: 10.3791/58162-v • 10:14 min • November 1st, 2018
November 1st, 2018 •
*1, *1, 2, 1
1Division of Renal-Electrolyte, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh
* These authors contributed equally

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Hovedfokuset for denne protokollen er effektivt isolere levedyktig primære glomeruli kulturer med minimal forurensninger for bruk i en rekke nedstrøms programmer. De isolerte glomeruli kan beholde strukturelle relasjoner mellom komponent celletyper og kultivert ex vivo for en kort tid.

Tags

Medisin problemet 141 nyre foten prosessen Effacement Glomerular skader Proteinuria Glomerular permeabilitet Protamine Sulfate nyre patologi Glomerular sykdom
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