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Journal / Immunology and Infection / Kvantitativ PCR av T7 Bacteriophage fra Biopanning
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantitativ PCR av T7 Bacteriophage fra Biopanning
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Kvantitativ PCR av T7 Bacteriophage fra Biopanning

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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En reproduserbare, nøyaktig og tid effektiv kvantitative PCR (qPCR) metode for å liste opp T7 bacteriophage er beskrevet her. Protokollen tydelig beskriver phage genomisk DNA forberedelse, PCR reaksjon forberedelse, qPCR sykling forhold og qPCR dataanalyse.

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Immunologi og infeksjon problemet 139 T7 phage phage genomisk DNA qPCR plakk analysen begrense av deteksjon kvantifisering utvalg
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