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Journal / Immunology and Infection / PCR quantitative du bactériophage T7 de criblage
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Immunology and Infection

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JoVE Journal Immunology and Infection
PCR quantitative du bactériophage T7 de criblage
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PCR quantitative du bactériophage T7 de criblage

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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Reproductible, précise et temps efficace quantitative PCR (qPCR) décrit une méthode pour énumérer bactériophage T7 est ici. Le protocole décrit clairement la préparation d’ADN génomique de phage, préparation de réaction PCR, qPCR conditions cyclisme et analyse de données de qPCR.

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Immunologie et Infection numéro 139 bactériophage T7 phage ADN génomique qPCR essai de plaque limite de détection gamme de quantification
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