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Journal / Biochemistry / 植物细胞悬浮液中咖啡因的提取、酶活性及咖啡因合成酶基因的表达…
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Biochemistry

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JoVE Journal Biochemistry
植物细胞悬浮液中咖啡因的提取、酶活性及咖啡因合成酶基因的表达
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植物细胞悬浮液中咖啡因的提取、酶活性及咖啡因合成酶基因的表达

Article DOI: 10.3791/58166-v • 09:11 min • October 2nd, 2018
October 2nd, 2018 •
1, 1, 1, 1, 2, 1
1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán

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本协议描述了一种高效的方法, 用于提取和定量的咖啡因在细胞悬浮液中的表达水平和评价咖啡因合酶酶活性的实验过程。编码这种酶的基因。

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生物化学 问题 140,咖啡咖啡 细胞悬浮 咖啡因提取 咖啡因合酶活性 CSS1基因表达 薄层密度 次生代谢产物
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