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Journal / Biochemistry / Koffein udvinding, enzymatisk aktivitet og…
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Biochemistry

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JoVE Journal Biochemistry
Koffein udvinding, enzymatisk aktivitet og genekspression af koffein syntase fra plante celle suspensioner
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Koffein udvinding, enzymatisk aktivitet og genekspression af koffein syntase fra plante celle suspensioner

Article DOI: 10.3791/58166-v • 09:11 min • October 2nd, 2018
October 2nd, 2018 •
1, 1, 1, 1, 2, 1
1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán

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Denne protokol beskriver en effektiv metode til udvinding og kvantificering af koffein i celle suspensioner af C. arabica L. og en eksperimenterende proces for at vurdere den enzymatiske aktivitet af koffein syntase med udtryk niveau af det gen, der koder dette enzym.

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Biokemi spørgsmål 140 Coffea arabica celle suspensioner koffein udvinding koffein syntase aktivitet CSS1 genekspression TLC-densitometri sekundære metabolitter
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