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JoVE Journal Biochemistry
Koffein utvinning, enzymaktivitet och genuttryck av koffein syntas från växten cellsuspensioner
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Koffein utvinning, enzymaktivitet och genuttryck av koffein syntas från växten cellsuspensioner

Article DOI: 10.3791/58166-v • 09:11 min • October 2nd, 2018
October 2nd, 2018 •
1, 1, 1, 1, 2, 1
1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán

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Det här protokollet beskriver en effektiv metod för utvinning och kvantifiering av koffein i cellsuspensioner C. arabica l. och en experimentell process för att utvärdera den enzymatiska aktiviteten av koffein syntas med uttryck den gen som kodar detta enzym.

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Biokemi fråga 140 Coffea arabica cellsuspensioner koffein utvinning koffein synthase aktivitet CSS1 genuttryck TLC-densitometry sekundära metaboliter
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