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Journal / Biochemistry / Kovalente immobilisering af proteiner for enkelt…
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Biochemistry

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JoVE Journal Biochemistry
Kovalente immobilisering af proteiner for enkelt molekyle kraft spektroskopi
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Kovalente immobilisering af proteiner for enkelt molekyle kraft spektroskopi

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Denne protokol beskriver den kovalente immobilisering af proteiner med en heterobifunctional silan kobling agent til siliciumoxid overflader designet til atomic force mikroskopi baseret enkelt molekyle kraft spektroskopi som er eksemplificeret ved den samspillet mellem RrgA (pilus-1 spids adhesin af S. pneumoniae) med fibronektin.

Tags

Biokemi spørgsmålet 138 kovalente protein immobilisering overflade silanisering heterobifunctional PIND reagens EDC/NHS kemi primære Amin atomic force mikroskopi AFM enkelt molekyle kraft spektroskopi SMF'ER
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