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Journal / Biochemistry / Imobilização covalente de proteínas para a…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Imobilização covalente de proteínas para a espectroscopia de força única molécula
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Journal (Biochemistry)
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
 
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Imobilização covalente de proteínas para a espectroscopia de força única molécula

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Este protocolo descreve a imobilização covalente de proteínas com um heterobifunctional agente de acoplamento silano para superfícies de óxido de silício projetado para a espectroscopia de força força atômica microscopia com base única molécula que é exemplificado a interação de RrgA (adesina pilus-1 ponta de S. pneumoniae) com fibronectina.

Tags

Bioquímica edição 138 imobilização covalente de proteínas superfície silanização heterobifunctional PEG reagente química EDC/NHS amina primária atomic force microscopia AFM única força espectroscopia molecular SMFS
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