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Journal / Biochemistry / Proteinler kovalent immobilizasyon için tek…
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Biochemistry

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JoVE Journal Biochemistry
Proteinler kovalent immobilizasyon için tek molekül kuvvet spektroskopisi
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Proteinler kovalent immobilizasyon için tek molekül kuvvet spektroskopisi

Article DOI: 10.3791/58167-v • 11:13 min • August 20th, 2018
August 20th, 2018 •
1,2,3, 2, 1,3, 3, 2, *4, *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen
* These authors contributed equally

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Bu iletişim kuralı ile örneklenir bir heterobifunctional silane Bağlantı Aracısı atomik kuvvet mikroskobu dayalı tek molekül kuvvet spektroskopisi için tasarlanmış silikon-oksit yüzeyler için proteinlerin immobilizasyon kovalent açıklar RrgA etkileşim (pilus-1 İpucu adhesin, S. pneumoniae) fibronektin ile.

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Biyokimya sayı 138 kovalent protein immobilizasyon yüzey silanization heterobifunctional PEG reaktif EDC/NHS kimya birincil Amin atomik kuvvet mikroskobu AFM tek molekül kuvvet spektroskopisi SMFS
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