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JoVE Journal Bioengineering
Ácido hialurônico baseado hidrogel para 3-Dimensional cultura de células de Glioblastoma derivado de paciente
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Ácido hialurônico baseado hidrogel para 3-Dimensional cultura de células de Glioblastoma derivado de paciente

Article DOI: 10.3791/58176-v • 08:35 min • August 24th, 2018
August 24th, 2018 •
1, 1, 1, 2
1Department of Bioengineering, University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute, University of California, Los Angeles

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Aqui, apresentamos um protocolo para cultura tridimensional de células de glioblastoma paciente-derivado dentro ortogonalmente sintonizáveis biomateriais projetados para imitar a matriz do cérebro. Essa abordagem fornece uma em vitro, plataforma experimental que mantém muitas características de na vivo glioblastoma células tipicamente perdidas na cultura.

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Bioengenharia edição 138 ácido hialurônico cultura 3D glioblastoma microambiente matriz extracelular biomaterial mechanobiology resistência a drogas
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